Senior/Research Associate, Functional Genomics

NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a new therapeutic mechanism to restore regenerative potential in aged and diseased cells. We leverage functional genomics, pooled perturbation screening, and machine learning models to unravel the biology of epigenetic aging and disease using experiments of unprecedented scale.

NewLimit is seeking a Senior/Research Associate with experience in molecular biology to join our Epigenetic Editing group. Pooled screening technologies are at the heart of our discovery engine, allowing us to rapidly search for epigenetic reprogramming therapies that restore cell function and reduce the burden of disease.

As a member of our team, you will:

• Collaborate with our Single Cell Technology team to implement large-scale, single cell perturbation experiments with custom chemistries
• Build recombinant DNA vectors by synthesis and cloning to enable large-scale single cell perturbation experiments
• Prepare, titer, and apply viral vectors (lentiviral, AAV) to transduce mammalian cell lines and primary cells
• Contribute to scaling and improving pooled single cell perturbation screens

• Bachelor’s degree in cell biology, molecular biology, or a related natural science or industry experience
• 2+ years of work experience in a cell or molecular biology laboratory (academic or industry)
• Experience with molecular cloning, nucleic acid analysis (RT-qPCR, gel electrophoresis), and related molecular biology methods
• Experience with mammalian cell culture

• Experience with next-generation sequencing assays and functional genomics
• Experience with FACS or flow cytometry
• Computational skills for next-generation sequencing analysis (Python programming, Unix)
• Experience with single cell genomics methods
• Experience with epigenetic reprogramming methods for cell fate engineering or targeted epigenetic editing