Research Technician Skilled In Mouse Work

The primary objective of the position is to provide research support to NHGRI scientists by per forming a variety of assignments involving the application of a full range of cell biology related laboratory methods, procedures and techniques associated with NIH biomedical research environment.

 

 

-Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.

-Collaboratively work with mouse models of FSASD and GNE in NHGRI animal facility, activities include mouse breeding, observation, tissue collection, testing of therapeutics and documentation of breeding/tissue collection logistics.

-Provide research support to NHGRI scientists with planning, developing and implementing la-boratory experiments and protocols; assist in coordinating communication with the Research Consortium on Free Sialic Acid Storage Disorder (FSASD).

-Perform laboratory technologies pertaining to molecular biology, biochemistry, glycobiology and cell biology. Such techniques include sterile cell culture, PCR, qRT-PCR, DNA/RNA as-says, cloning, protein assays, glycobiology tools, lectin technologies, antibody techniques.

-Operate a variety of laboratory instruments, including but not limited to (confocal)-microscopes, spectrophotometer/plate reader, other imaging equipment.

-Operates laboratory machines and instruments; and prepares specimens and equipment for use.

-Work with staff on processing specimens for embedding according to protocols. 

-Maintain inventory system and sample storage according to outlined guidelines. 

-Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/ immunohistochemistry.

-Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.

- Perform primary cell culture techniques and immunohistochemistry. 

 

- Biosafety Level 1 (BSL 1) 

- Degree in Biology or related field  

- PowerPoint, Excel, Word, R 

- Animal Handling: Mouse

- Manuscript writing

- Tissue Sectioning

- Data Presentation

- Primary cell culture

- Western Blotting