Senior Research Associate, Metabolism

NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a new therapeutic mechanism to restore regenerative potential in aged and diseased cells. We leverage functional genomics, pooled perturbation screening, and machine learning models to unravel the biology of epigenetic aging and disease using experiments of unprecedented scale.

NewLimit is seeking a Senior Research Associate with experience in liver biology or in vivo physiology to join our Metabolism team. Our Metabolism team is developing reprogramming medicines to restore youthful function in aged hepatocytes in the context of metabolic disease. In this role, you will help develop epigenetic editing tools, functional assays, and model systems to measure the effectiveness of our therapeutic candidates.

• →Collaborate with our Single Cell Technology, Epigenetic Editing, and Predictive Modeling teams to design, build, and test epigenetic reprogramming interventions for aged and diseased hepatocytes
• →Perform experiments in preclinical animal models of liver disease
• →Conduct functional and phenotypic assays to evaluate the effects of reprogramming on hepatocyte biology in vivo
• →Deliver epigenetic reprogramming perturbations to hepatocytes in vivo using multiple transgenic manipulation methods (e.g. viral vectors, lipid nanoparticles)
• →Isolate murine and human hepatocytes by liver perfusion with downstream FACS analysis

• BS in cell biology, molecular biology, or a related natural science and 2+ years of work experience in a cell biology or animal physiology laboratory (academic or industry setting)
• Proficiency in animal handling and husbandry techniques for rodent models (mice or rats)
• Exceptional organizational skills, including detailed experimental record-keeping and the ability to manage multiple concurrent projects
• Experience with standard molecular biology techniques, including gel electrophoresis, nucleic acid purification, and PCR

• Experience working with mouse models of aging, disease and liver dysfunction
• Experience with in vivo manipulation of hepatocytes and liver perfusion techniques
• Experience with single-cell genomics or other functional genomics approaches
• Proficiency in molecular biology and molecular cloning methods