Research Lab Technician IV - Wake Forest Institute for Regenerative Medicine

1st

The ideal candidate will have experience in:

• Primary immune cell isolation, culture, and stimulation

• Flow cytometry and immunoassay execution (ELISA, ELISpot, multiplex cytokine)

• Hydrogel and biomaterial scaffold preparation and characterization

• Data collection and analysis for complex biological systems

This position offers unique opportunities to:

• Work with state-of-the-art organ-on-chip and microfluidic integration technology

• Contribute to New Approach Methodologies that reduce reliance on animal testing

• Participate in research that directly supports vaccine and medical countermeasure development

• Gain experience with engineered immune tissues, biosensing, and multi-OTE integrated systems

• Join a collaborative research environment at a leading regenerative medicine institute

The Wake Forest Institute for Regenerative Medicine (WFIRM) seeks a Research

Laboratory Technician IV to join the ImmuneChip program, which develops microfluidic organ tissue equivalent (OTE) platforms for vaccine efficacy testing. The position focuses on the lymph node OTE, an engineered immune tissue that supports antigen presentation and antibody production, and on its integration with bone marrow, muscle, and airway OTEs into a functional immune cascade. This position offers an opportunity to contribute to research that advances preclinical vaccine evaluation without

animal testing.

Under general supervision, the successful candidate will:

• Execute complex experimental protocols for lymph node OTE fabrication, maturation, and

functional testing, including hydrogel and scaffold-based tissue assembly

• Isolate and culture primary human immune and stromal cells, including B cells, T cells, dendritic

cells, fibroblastic reticular cells, and endothelial cells

• Conduct immune functional analyses including multiparameter flow cytometry, ELISA and

ELISpot, multiplex cytokine assays, antigen-specific and neutralizing antibody measurement, and

immunohistochemistry

• Support microfluidic culture, perfusion, and integration of the lymph node OTE with other tissue

modules, and participate in experimental design and optimization

• Provide technical guidance to other laboratory personnel

Bachelor's degree in the Biological and/or Physical Sciences with four years of related chemical/research experience. Master's degree in Biology or related Physical Science with two years of laboratory experience preferred.

Advocate Health offers a comprehensive suite of Total Rewards: benefits and well-being programs, competitive compensation, generous retirement offerings, programs that invest in your career development and so much more – so you can live fully at and away from work, including:

Advocate Health is the third-largest nonprofit, integrated health system in the United States, created from the combination of Advocate Aurora Health and Atrium Health. Providing care under the names Advocate Health Care in Illinois; Atrium Health in the Carolinas, Georgia and Alabama; and Aurora Health Care in Wisconsin, Advocate Health is a national leader in clinical innovation, health outcomes, consumer experience and value-based care. Headquartered in Charlotte, North Carolina, Advocate Health services nearly 6 million patients and is engaged in hundreds of clinical trials and research studies, with Wake Forest University School of Medicine serving as the academic core of the enterprise. It is nationally recognized for its expertise in cardiology, neurosciences, oncology, pediatrics and rehabilitation, as well as organ transplants, burn treatments and specialized musculoskeletal programs. Advocate Health employs 155,000 teammates across 69 hospitals and over 1,000 care locations, and offers one of the nation’s largest graduate medical education programs with over 2,000 residents and fellows across more than 200 programs. Committed to providing equitable care for all, Advocate Health provides more than $6 billion in annual community benefits.