Principal Research Associate, Analytical Development

eGenesis is a clinical-stage biotechnology company developing human-compatible engineered organs to address the severe global organ shortage. The Company’s proprietary genome engineering platform enables extensive, multiplex gene edits to remove key biological barriers, add protective human transgenes, and inactivate endogenous retroviruses. EGEN-2784, a genetically engineered porcine kidney, is the Company’s lead program and is currently being evaluated in a multi-patient Expanded Access study at MGH. eGenesis is headquartered in Cambridge, MA. 

 

We are seeking a highly motivated Principal Research Associate to join the Analytical Development team. This is an opportunity to develop analytical methods supporting gene-edited xenotransplantation products in a dynamic environment focused on molecular and cellular engineering. 

In this role, you will independently develop analytical assays from initial concept and feasibility through optimization, qualification, and transfer. Responsibilities include assessing transgene expression and biological function in genetically modified porcine cells, with a particular emphasis on developing robust, mechanism-relevant potency assays. The successful candidate will be expected to select appropriate assay formats and biological readouts, establish controls, understand critical assay variables, and solve complex development challenges with limited supervision. 

The position involves significant hands-on cell culture, including the isolation, growth, and maintenance of porcine primary cells. Methods may include cell-based functional assays, flow cytometry, ELISA, and real-time PCR. This is a full-time, laboratory-based position suited for a proactive, rigorous, collaborative scientist who is comfortable building new methods where established procedures may not yet exist. 

B.S. or B.A. in cell biology, immunology, biomedical engineering, or a related scientific discipline with 6-8 years of relevant industry experience, or an M.S. with 4-6 years of relevant industry experience.

Demonstrated experience independently developing analytical methods from initial concept and feasibility through optimization, qualification, and implementation.

Hands-on experience developing, optimizing, and troubleshooting assays that measure biological activity or potency.

Expertise in mammalian cell culture and assay development using flow cytometry and ELISA, including control strategy and troubleshooting.

Experience designing controlled experiments, interpreting multivariable data, evaluating assay performance, and establishing fit-for-purpose controls and acceptance criteria.

Ability to independently manage laboratory work across concurrent projects, document and communicate scientific results clearly, and collaborate effectively across functions.