Postdoctoral Fellow-MSH

Dr. Sahoo’s laboratory studies epitranscriptomic- andexosomes-mediated mechanisms in pathologies and therapeutics of the heart. The projects involve a broad array of advanced techniques in exosomes and epitranscriptome biology, including MeRIP sequencing, confocal, super-resolution and electron microscopy, small non-coding RNAs, stable-isotope-labeling mass spectrometry, in vitro and in vivo imaging, live imaging of exosomes, microfluidics isolation, bionanotechnology, systems genetics and small and large animal models of heart failure to address scientific questions in innovative ways.

Position Description: The position is for specialized research professionals who, under the direction of Dr. Sahoo, will provide direct professional support of all research activities in the laboratory. The positions involve familiarity with processing human, swine and rodent tissue specimens, maintaining an up to date database, managing several laboratory activities and working with other members of the team to guide and provide necessary support as needed. The responsibilities also include maintaining laboratory protocols, safety compliance, grant writing, training new employees, and overall laboratory management.

Roles and Responsibilities:

Specialized laboratory technique requirements for the project:
Cell and tissue culture, Imaging, flow cytometry, histology of the heart, echocardiography, MTT assay to assess metabolic activity, Western blot, PCR, RNA sequencing and data analysis, exosomes isolation, m6A assays, MeRIP sequencing, data anlysis, bioinformatics, cross linking, RNA_protein interactions and other molecular biology techniques.

Other significant laboratory techniques specific for the project:
ELISA, genotyping, transduction, transfection, bacterial transformation, cell proliferation assay, microbial culture, transfection, AAV transduction, miniprep and maxiprep, AAV isolation and purification, IVIS imaging, proficiency in q-PCR, RT-PCR, RNA extraction, cDNA synthesis, DNA/RNA gels, quantification, western blot, immunocytochemistry, fluorescence microscope, agarose gel electrophoresis, spectrophotometer,

Animal Handling: Mice handling, intraperitonial injection, Tissue processing, bone marrow extraction to derive dendritic cells, T-Cell isolation from PBMC by using MACS.

We are looking for candidates with an MS/PhD degree with several years of research experience in a laboratory setting in molecular biology, cell culture and animal handling.

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