Research Associate

Responsible for processing patient samples for RNA and DNA extraction and perform downstream assays to include qPCR.

• Follows SOPs for scientific purposes with guidance
• Uses professional concepts in accordance with company objectives to solve routine to moderately difficult problems by performing scientific functions in collaboration with others
• Isolation and cryopreservation of intact RNA, miRNA and DNA from clinical patient samples of all tissue and types
• Quantification and QC evaluation of nucleic acid samples including Agilent Tapestation, spectrophotometric assays (Nanodrop), Ribogreen and Picogreen assays
• Plans and conducts DNA and RNA purification projects at various scales and from different material types with supervision
• Performs QC analyses (RNA/DNA integrity analysis, Real-Time PCR) to ensure that results meet specifications with supervision
• Works on problems of routine to moderate scope in which the analysis of situation or data requires review of identifiable factors
• Exercises judgment within defined practices and procedures to determine appropriate action.
• Assists with writing/developing new SOPs
• Assists with evaluation new technologies and beta testing
• Monitors assays and troubleshoots with help from supervisor
• Prepare all reagents used in lab
• Record and update information in Batch Records
• Build and perform first key of data collection sheets
• Perform equipment preventive maintenance
• Order and inspect lab supplies
• Carry out other duties/projects as assigned

• Bachelor’s Degree (Biology, Chemistry or Life Sciences) with 2 years’ relevant laboratory experience or equivalent combination of education and experience

• DNA RNA purification
• qPCR/qRT-PCR
• Molecular-based assays quantitation assays such as Ribogreen, Picogreen and spectrophotometric assessment
• Must be proficient using Microsoft office, specifically with Excel.  Familiar with Softmax Pro
• Requires good attention to detail, excellent documentation skills and willingness to work well in a group environment
• Ability to lift and carry between 1-15 lbs.; Ability to lift up to 50 lbs. overhead with assistance; frequently required to sit, grasp, and use right/left finger dexterity; May be exposed to a cold freezer environment (Freezer temperatures range between -20ºC -196ºC)
• You will read, write, speak fluently and comprehend the English language

• Experience with multiple automated nucleic acid extraction platforms
• Use of at least one Laboratory Information Management System (LIMS)
• QC of nucleic acid such as Agilent Tapestation or other protocols