Scientist I (Gene Therapy/ Cell Cloning)

Our client, a world leader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA. Job Duration: Long Term Contract (Possibility Of Extension) Pay Rate : $46.43/hr on W2 Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays. Job Responsibilities: · Design and clone expression vectors and IVT donor plasmids to support research initiatives. · Execute DNA and mRNA transfections in mouse and human cell lines. · Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting. · Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides Required Qualifications: Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field. Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT. Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR. Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes). Desired proficiency in Snapgene, Prism, and MS Office. If interested, please send us your updated resume at hr@dawarconsulting.com/akansha@dawarconsulting.com