18d ago

Research Opportunities - McDonnell Genome Institute

Washington University Medical Campusmid

Other

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Quick Summary

Key Responsibilities

For more about this project, visit https://genome.wustl.edu/the-resiliency-project-advancing-neurodegenerative-disease-research-at-mgi/. Working Conditions: Because some cell culture, differentiation,

Requirements Summary

Bachelor’s degree or combination of education and/or experience may substitute for minimum education.

Technical Tools

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ST. LOUIS, MO 63108

We are seeking a highly motivated scientist to join our functional genomics team at the Goldfarb Center for Neuronal Resiliency at the McDonnell Genome Institute, WashU Medicine. The Goldfarb Center for Neuronal Resiliency was established to identify new therapeutic targets and treatments for neurodegenerative diseases, particularly ALS and Parkinson’s disease.

Central to this effort are CRISPR screens that identify genes that make neurons more vulnerable or resilient to stress. These screens will use human induced pluripotent stem cell (iPSC)-derived neuronal models of neurodegenerative diseases. Work in the lab also integrates CRISPR perturbations with phenotypic assays and molecular readouts using flow cytometry, high content imaging, next-generation sequencing, and single-cell, spatial, and in situ profiling.

About The Goldfarb Center for Neuronal Resiliency

Led by Drs. Jeff Milbrandt and Rob Mitra, The Goldfarb Center for Neuronal Resiliency is a highly collaborative and interdisciplinary research environment integrating functional genomics, stem cell engineering, neuroscience, high throughput imaging, next-generational sequencing, artificial intelligence, and drug discovery. Our work is inspired by the observation that neurodegeneration is strikingly selective for specific neuronal populations. For example, in ALS, motor neurons degenerate while neighboring interneurons remain intact. Even within motor neurons, some subtypes are highly vulnerable, while others are resilient. We believe these differences in resiliency provide clues to new therapeutic strategies that have yet to be harnessed for drug development. Specifically, our long-term goal is to discover therapeutics that activate resiliency factors or inhibit vulnerability factors in diseased neurons in order to restore them to heath and reverse the progression of these debilitating diseases.

Responsibilities

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For more about this project, visit https://genome.wustl.edu/the-resiliency-project-advancing-neurodegenerative-disease-research-at-mgi/.

Because some cell culture, differentiation, and time-sensitive experimental workflows require procedures on specific days, the successful candidate must be able to accommodate occasional weekend work when required by experimental schedules.

This position works in a laboratory environment with potential exposure to biological and chemical hazards.

The individual must be physically able to wear protective equipment.

Base pay is commensurate with experience.

The above statements are intended to describe the general nature and level of work performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all job duties performed by the personnel so classified. Management reserves the right to revise or amend duties at any time.

Requirements

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Candidates with a bachelor's, master's degree, or PhD will be considered.

Substantial research experience in molecular biology, cell biology, genetics, genomics, neuroscience, bioengineering, or a related field.

Strong hands-on experience in mammalian cell culture and molecular biology.

Strong organizational skills and ability to manage multiple experiments and priorities.

Strong written and verbal communication skills.

Bachelor’s degree or combination of education and/or experience may substitute for minimum education. No additional education unless stated elsewhere in the job posting.

No specific certification/professional license is required for this position.

No additional certification/professional licenses unless stated elsewhere in the job posting.

No specific work experience is required for this position. No additional work experience unless stated elsewhere in the job posting.

Not Applicable Communication, Laboratory Research, Organizing, Recordkeeping, Working Independently

A driver's license is not required for this position.

About the Role

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Nice to Have

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Experience in one or more of the following areas would be highly desirable:

CRISPR/Cas genome editing, CRISPR screening, or functional genomics.

Human iPSC culture and differentiation, particularly neuronal differentiation.

Lentiviral vector production and transduction.

Flow cytometry and fluorescence-activated cell sorting (FACS).

Reporter assay development and engineered stable cell lines.

qRT-PCR and Western blotting.

DNA/RNA purification, PCR, cloning, and other molecular biology techniques.

Next-generation sequencing and sequencing-library preparation.

Experience with every technique listed above is not required. Candidates with a strong foundation in mammalian cell and molecular biology, demonstrated scientific independence, and the ability to rapidly learn new technologies are encouraged to apply.

Final appointment and title will be commensurate with the candidate's qualifications and experience.

Base pay is commensurate with experience.

The salary range reflects base salaries paid for positions in a given job grade across the University. Individual rates within the range will be determined by factors including one's qualifications and performance, equity with others in the department, market rates for positions within the same grade and department budget.

For frequently asked questions about the application process, please refer to our External Applicant FAQ.

If you are unable to use our online application system and would like an accommodation, please email CandidateQuestions@wustl.edu or call the dedicated accommodation inquiry number at 314-935-1149 and leave a voicemail with the nature of your request.

All qualified individuals must be able to perform the essential functions of the position satisfactorily and, if requested, reasonable accommodations will be made to enable employees with disabilities to perform the essential functions of their job, absent undue hardship.

All external candidates receiving an offer for employment will be required to submit to pre-employment screening for this position. The screenings will include criminal background check and, as applicable for the position, other background checks, drug screen, an employment and education or licensure/certification verification, physical examination, certain vaccinations and/or governmental registry checks. All offers are contingent upon successful completion of required screening.

What We Offer

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Personal

✓Up to 22 days of vacation, 10 recognized holidays, and sick time.

✓Competitive health insurance packages with priority appointments and lower copays/coinsurance.

✓Take advantage of our free Metro transit U-Pass for eligible employees.

✓WashU provides eligible employees with a defined contribution (403(b)) Retirement Savings Plan, which combines employee contributions and university contributions starting at 7%.

✓Wellness challenges, annual health screenings, mental health resources, mindfulness programs and courses, employee assistance program (EAP), financial resources, access to dietitians, and more!

✓We offer 4 weeks of caregiver leave to bond with your new child. Family care resources are also available for your continued childcare needs. Need adult care? We’ve got you covered.

✓WashU covers the cost of tuition for you and your family, including dependent undergraduate-level college tuition up to 100% at WashU and 40% elsewhere after seven years with us.

Washington University in St. Louis is committed to the principles and practices of equal employment opportunity. It is the University’s policy to provide equal opportunity and access to persons in all job titles without regard to race, ethnicity or shared ancestry, color, national origin, citizenship (where prohibited by federal law), age, religion, sex, sexual orientation, gender identity or expression, disability, protected veteran status, or genetic information. As a federal contractor, the university maintains affirmative action programs to implement its equal employment opportunity policy for individuals with disabilities and protected veterans, in accordance with Section 503 of the Rehabilitation Act of 1973, as amended, and the Vietnam Era Veterans’ Readjustment Assistance Act of 1974 (VEVRAA), as amended.