Senior/Research Associate, mRNA Engineering

NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a new therapeutic mechanism to restore regenerative potential in aged and diseased cells. We leverage functional genomics, pooled perturbation screening, and machine learning models to unravel the biology of epigenetic aging and disease using experiments of unprecedented scale.

We are seeking a Research Associate to join our mRNA Engineering team. In this role, you will generate the mRNAs that power NewLimit's research programs, executing the workflow from DNA template through in vitro transcription (IVT) and downstream purification. You will produce research grade mRNA materials, keep rigorous records of how each batch was made, and work closely with scientists across our research and Delivery Sciences teams. This is a lab-based role ideal for an early-career scientist who wants to build deep expertise in mRNA process development in a fast-paced research environment. You will receive hands-on training from expert scientists and see the direct impact of your work on our programs.

As a member of our team, you will:

• Execute established protocols for DNA template preparation, in vitro transcription, and downstream purification to generate research-grade mRNA
• Run routine analytical assays to assess mRNA integrity and purity
• Under the guidance of senior scientists, help troubleshoot production issues and contribute to experiments that optimize yield, purity, and recovery

• Maintain detailed, accurate records of all experiments in Electronic Lab Notebooks
• Follow and help maintain SOPs; contribute to drafting and updating protocols as workflows evolve
• Manage raw material and mRNA inventory, including sample tracking, storage, and reagent ordering
• Maintain lab equipment and follow lab safety practices

• Work closely with Delivery Sciences and research teams to coordinate material needs and hand off mRNA for downstream studies
• Present experiment results at team meetings

• B.S. in Life Sciences, Chemistry, Engineering, or a related field with 1-2 years of relevant full-time industry or academic research experience, or M.S. in Life Sciences
• Hands-on experience with molecular biology techniques such as cloning, nucleic acid purification, PCR, and gel electrophoresis
• Strong attention to detail and good documentation practices
• Ability to execute protocols accurately and manage multiple tasks efficiently
• Excellent communication and organizational skills
• Interest in mRNA process science and eagerness to learn new techniques

• Experience producing mRNA through IVT and downstream purification
• Familiarity with plasmid and mRNA analytics such as HPLC, UV-Vis, and TapeStation/CGE
• Experience with plasmid DNA preparation and linearization
• Exposure to column-based purification and tangential flow filtration (TFF)
• Familiarity with automation liquid handling-based methods for mRNA purification, including experience operating such as Hamiltons and Tecans