Senior/Research Associate, Single Cell Technology

NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a new therapeutic mechanism to restore regenerative potential in aged and diseased cells. We leverage functional genomics, pooled perturbation screening, and machine learning models to unravel the biology of epigenetic aging and disease using experiments of unprecedented scale.

• Measure the biodistribution, pharmacokinetics & efficacy of reprogramming medicines using traditional molecular assays as well as frontier genomics methods
• Design and execute experiments involving the isolation, culture, and processing of cells, tissues, and organs for molecular profiling
• Collaborate with our Epigenetic Editing team to perform large-scale, single cell perturbation experiments with custom chemistries
• Perform state-of-the-art single cell profiling experiments to map epigenetic features of age-related disease and evaluate interventions

• Bachelor’s degree in cell biology, molecular biology, or a related natural science or industry experience
• 2+ years of work experience in a cell or molecular biology laboratory (academic or industry)
• Experience with standard molecular biology workflows (e.g., nucleic acid extraction, qPCR, ELISA, NGS library construction, library quantification and QC)
• Experience with necropsy, organ/tissue collection, primary tissue dissociation, mammalian cell culture and sample processing (e.g. single cell suspension preparation, nuclei isolation, fixation)

• Experience with in vivo animal handling
• Experience with single cell genomics methods (such as 10x Genomics, Parse Bio, Perturb-seq/CROProp-seq or similar)
• Experience troubleshooting or optimizing NGS workflows.
• Experience with flow cytometry sorting for single-cell preparations.