Senior Research Associate - Technology Development

Profluent is seeking a creative, passionate, and detail-oriented individual to join our Technology Development team and contribute to the development and application of high-throughput workflows for genome editing.

This role revolves around execution and continual development of HTP cloning and mammalian transfection experiments. You will work at the intersection of biology, automation, and data to advance cutting-edge research. You will identify, prioritize, and drive impactful projects and contribute to the conception, design, analysis, and interpretation of experiments. You will bring creative, literature-informed problem-solving to early-stage conceptualization and troubleshooting, and apply a detail-oriented, data-driven mindset during late-stage optimization.
 

As part of the team, you will excel as both an individual contributor and a collaborator, actively supporting shared goals while learning from and contributing to new technical and conceptual areas. Your adaptability and curiosity will help advance Profluent’s mission to develop transformative gene-editing technologies.

• →Develop, execute, and automate arrayed and pooled mammalian cell-based gene editing assays
• →Design and execute low and high throughput plasmid cloning campaigns for construction of DNA libraries
• →Leverage variety of readouts, including plate-based assays, flow cytometry, and next generation sequencing (NGS)
• →Coordinate with manager and stakeholder to prioritize and design impactful projects 
• →Perform mammalian cell culture and transfection
• →Precisely track and document experimental samples, assay data, and results
• →Analyze experimental data and interpret results
• →Present experimental results to colleagues in team and cross-team meetings
• →Contribute to lab maintenance and adhere to EHS standards 

• BS and in Molecular Biology, Biochemistry, Cell Biology, or a related field and 4 years or MS in in Molecular Biology, Biochemistry, Cell Biology, or a related field and 2 years of relevant industry research experience 
• Extensive, hands-on experience with mammalian and bacterial cell culture and molecular biology
• Excellent communication skills and the ability to work collaboratively across interdisciplinary teams
• Strong organizational skills to accurately track laboratory samples and experimental data

Legal authorization to work in the United States is required. In compliance with federal law, all persons hired must verify their identity and work eligibility and complete the required employment verification form upon hire.

• Experience with CRISPR and/or mammalian cell-based gene editing assays
• Experience with next generation sequencing wet lab and computational workflows/analysis
• Experience with high throughput screening workflows
• Experience with flow cytometry 
• Experience with process development and and structured collaboration
• Experience processing and analyzing large experimental datasets (i.e. Python or JMP)